This study addresses the critical need for high signal-to-noise ratio in optical detection methods for biological sample discrimination under low-photon-flux conditions to ensure accuracy without compromising sample integrity. We explore polarization-based probing, which often excels over intensity modulation when assessing a specimen's morphology. Leveraging non-classical light sources, our approach capitalizes on sub-Poissonian photon statistics and quantum correlation-based measurements. We present a novel, highly sensitive method for probing single-layer cell cultures using entangled photon pairs. Our approach demonstrates potential for monolayer cell analysis, distinguishing between two types of monolayer cells and their host medium. The experimental results highlight our method's sensitivity, showcasing its potential for biological sample detection using quantum techniques, and paving the way for advanced diagnostic methodologies.
This study addresses the critical need for high signal-to-noise ratio in optical detection methods for biological sample discrimination under low-photon-flux conditions to ensure accuracy without compromising sample integrity. We explore polarization-based probing, which often excels over intensity modulation when assessing a specimen's morphology. Leveraging non-classical light sources, our approach capitalizes on sub-Poissonian photon statistics and quantum correlation-based measurements. We present a novel, highly sensitive method for probing single-layer cell cultures using entangled photon pairs. Our approach demonstrates potential for monolayer cell analysis, distinguishing between two types of monolayer cells and their host medium. The experimental results highlight our method's sensitivity, showcasing its potential for biological sample detection using quantum techniques, and paving the way for advanced diagnostic methodologies.
Probing the polarization response of an object with the well-acknowledged technique of optical polarimetry enables determination of the specimen's inner properties. Prominent examples include the measurement of the chirality of proteins and molecules [1] or the induced anisotropy of disease-affected cells and tissues [2]. Aiming for better signal-to-noise ratio, lower photon flux of the probing beam and non-local diagnostics, polarimetric approaches employing the quantum nature of light and entanglement are currently rapidly developing [3]–[6]. In the present work we study the experimental feasibility of the non-local identification of different polarizing samples by performing only few coincidence measurements [6] without necessity for full density matrix reconstruction of the quantum state.
With the advancement of non-classical light sources such as single-photon and entangled-photon sources, innovative microscopy based on quantum principles has been proposed for traditional microscopy. This paper introduces the experimental demonstration of a quantum polarization microscopic technique that incorporates a quantum-entangled photon source. Although the point that employs the variation in polarization angle due to reflection or transmission at the sample is similar to classical polarization microscopy, the method for constructing the image contrast is significantly different. The image contrast is constructed by the coincidence count of signal and idler photons. In the case that the coincidence count is recorded from both the signal and idler photons, the photon statistics resemble a thermal state, similar to the blackbody radiation, but with a significantly higher peak intensity in the second-order autocorrelation function at zero delay that is derived from the coincidence count, while, when the coincidence count is taken from either the signal or idler photon only, although the photon state exhibits a thermal state again, the photon statistics become more dispersive and result in a lower peak intensity of the autocorrelation function. These different thermal states can be switched by slightly changing the photon polarization, which is suddenly aroused within a narrow range of the analyzer angle. The autocorrelation function g2(0) at the thermal state exhibits a sensitivity that is three times higher compared to the classical coincidence count rate, and this concept can be effectively utilized to enhance the contrast of the image. One of the key achievements of our proposed method is ensuring a low power of illumination (in the order of Pico-joules) for constructing the image. In addition, the robustness without any precise setup is also favorable for practical use. This polarization microscopic technique can provide a superior imaging technique compared to the classical method, opening a new frontier for research in material sciences, biology, and other fields requiring high-resolution imaging.
With the advancement of non-classical light sources such as single-photon and entangled-photon sources, innovative microscopy based on the quantum principles has been proposed over traditional microscopy. This paper introduces the experimental demonstration of a quantum polarization microscopic technique that incorporates a quantum-entangled photon source. Although the point that employs the variation of polarization angle due to reflection or transmission at the sample is similar to classical polarization microscopy, the method for constructing image contrast is significantly different. Image contrast is constructed by the coincidence count of signal and idler photons. In the case that coincidence count is recorded from both the signal and idler photons, the photon statistics resemble a thermal state, similar to the blackbody radiation, but with a significantly higher peak intensity in the second order autocorrelation function at zero delay that is derived from coincidence count. While, when the coincidence count is taken from either the signal or idler photon only, though the photon state exhibits a thermal state again, the photon statistics become more dispersive and result in a lower peak intensity of the autocorrelation function. These different thermal states can be switched by slightly changing the photon polarization, which is suddenly aroused within narrow range of analyzer angle. This polarization microscopic technique can provide a superior imaging technique compared to the classical method, opening a new frontier for research in material sciences, biology, and other fields requiring high-resolution imaging.
Detecting object with low reflectivity embedded within a noisy background is a challenging task. Quantum correlations between pairs of quantum states of light, though are highly sensitive to background noise and losses, offer advantages over traditional illumination methods. Instead of using correlated photon pairs which are sensitive, we experimentally demonstrate the advantage of using heralded single-photons entangled in polarization and path degree of freedom for quantum illumination. In the study, the object of different reflectivity is placed along the path of the signal in a variable thermal background before taking the joint measurements and calculating the quantum correlations. We show the significant advantage of using non-interferometric measurements along the multiple paths for single photon to isolate the signal from the background noise and outperform in detecting and ranging the low reflectivity objects even when the signal-to-noise ratio is as low as 0.03. Decrease in visibility of polarization along the signal path also results in similar observations. This will have direct relevance to the development of single-photon based quantum LiDAR and quantum imaging.
Polarimetric imaging, a technique that captures the invisible polarization-related properties of given materials, has broad applications from fundamental physics to advanced fields such as target recognition, stress detection, biomedical diagnosis and remote sensing. The introduction of quantum sources into classical imaging systems has demonstrated distinct advantages, yet few studies have explored their combination with polarimetric imaging. In this study, we present a quantum polarimetric imaging system that integrates polarization-entangled photon pairs into a polarizer-sample-compensator-analyzer-typed polarimeter. Our system visualizes the birefringence properties of a periodical-distributed anisotropic material under decreasing illumination levels and diverse disturbing light sources. Compared to the classical system, the quantum approach reveals the superior sensitivity and robustness in low-light conditions, particularly useful in biomedical studies where the low illumination and non-destructive detection are urgently needed. The study also highlights the nonlocality of entangled photons in birefringence measurement, indicating the potential of quantum polarimetric system in the remote sensing domain.
Polarimetric imaging, a technique that captures the invisible polarization-related properties of given materials, has broad applications from fundamental physics to advanced fields such as target recognition, stress detection, biomedical diagnosis and remote sensing. The introduction of quantum sources into classical imaging systems has demonstrated distinct advantages, yet few studies have explored their combination with polarimetric imaging. In this study, we present a quantum polarimetric imaging system that integrates polarization-entangled photon pairs into a polarizer-sample-compensator-analyzer (PSRA)-type polarimeter. Our system visualizes the birefringence properties of a periodical-distributed anisotropic material under decreasing illumination levels and diverse disturbing light sources. Compared to the classical system, the quantum approach reveals the superior sensitivity and robustness in low-light conditions, particularly useful in biomedical studies where the low illumination and non-destructive detection are urgently needed. The study also highlights the nonlocality of entangled photons in birefringence measurement, indicating the potential of quantum polarimetric system in the remote sensing domain.