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Quantum Effects and Measurement Techniques in Biology and Biophotonics

Fluorescence lifetime measurement based on quantum sensing

Giuseppe Di Blasio, Federico Simoni, Lorenzo Uboldi, Ilaria Bargigia, Daniele Faccio, Giulio Cerullo, Antonio Pifferi, Cosimo D’Andrea

Published Mar 4, 2026• 0 citations
Publisher

Abstract

Quantum light sources, such as entangled photons, offers unique advantages for bio-sensing of light sensitive samples, providing background free and high SNR measurements at ultra-low photon flux. We built a modular set-up based on SPDC entangled photon pairs generation by using a pulsed laser and several single-photon detectors with different time resolution and quantum efficiency to investigate fluorescent complexes. This toolkit paves the way for minimally invasive fluorescence lifetime sensing towards next generation in vivo studies.

DOI
10.1117/12.3080946

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Quantum light sources, such as entangled photons, offers unique advantages for bio-sensing of light sensitive samples, providing background free and high SNR measurements at ultra-low photon flux. We built a modular set-up based on SPDC entangled photon pairs generation by using a pulsed laser and several single-photon detectors with different time resolution and quantum efficiency to investigate fluorescent complexes. This toolkit paves the way for minimally invasive fluorescence lifetime sensing towards next generation in vivo studies.
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