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International Conference on Ultrafast Phenomena

Fluorescence detection of femtosecond accumulated photon echo

Kiyoshi Uchikawa, H. Ohsawa, S. Saikan

Published Jan 1, 1990• 0 citations
Publisher

Abstract

Generally, the photon echo measurement in opaque and scattering materials is difficult since the scattered pump beam overloads the detector. In this sense, a different modulation technique which does not induce the intensity modulation of the pump beam, is desirable for the scattering materials. We previously reported [1] that phase modulation method is also applicable to the measurement of accumulated photon echo. This technique has been much improved, and we have succeeded the fluorescence detection of the femtosecond accumulated photon echo.

DOI
10.1364/up.1990.wc4

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Generally, the photon echo measurement in opaque and scattering materials is difficult since the scattered pump beam overloads the detector. In this sense, a different modulation technique which does not induce the intensity modulation of the pump beam, is desirable for the scattering materials. We previously reported [1] that phase modulation method is also applicable to the measurement of accumulated photon echo. This technique has been much improved, and we have succeeded the fluorescence detection of the femtosecond accumulated photon echo.
A new method for waveform measurement of photon echoes using a modified Michelson interferometer has been developed. The experimental setup uses collinear geometry, and photon echo signals are detected using a double-phase modulation technique. The amplitude and phase of photon echoes and those of excitation pulses are simultaneously measured by this technique. The method has been applied to a photon echo experiment on a dye solution. The results are explained using a stochastic modulation model with an accumulation effect of population grating.
The fluorescence detection technique of incoherent accumulated photon echo is applied to the investigation of animal and human tissues. The correlation between the kind of animal tissue and the dephasing time is determined. The dephasing times of normal and cancerous human tissues are measured by this technique. The cancer tissues exhibit shorter dephasing times than the normal tissues, and this is interpreted as being caused by the large number of conformational substates of cancer tissues. The dephasing time of a benign-tumor tissue is also measured and shows a value similar to that of the normal tissue. The dephasing time of a metastasizing cancer region is also measured. The applicability of photon-echo spectroscopy to diagnosis is noted.