First-generation long-distance quantum repeater networks require quantum memories capable of interfacing with telecom photons to perform quantum-interference-mediated entanglement generation operations. The ability to demonstrate these interconnections using real-life fiber connections in a long-distance setting is paramount to realize a scalable quantum internet. Here we address these significant challenges by observing Hong-Ou-Mandel (HOM) interference between indistinguishable telecom photons produced in two independent room temperature quantum memories, separated by a distance of 158 km. We obtained interference visibilities after long-distance propagation of
V=(38±2)% for single-photon level experimental inputs. This first-of-its-kind quantum network prototype connecting quantum laboratories in Stony Brook University and Brookhaven National Laboratory is envisioned to evolve into a large-scale memory-assisted entanglement distribution quantum network, the basis for inter-city quantum communication.