Identifying and Distinguishing Single-molecule Based on the Fluorescence Photon Statistic Measurement
Peng Shuang-Yan, Huang Tao
Identifying and distinguishing single-molecule by fluorescence photon statistic measuring have been widely applied in physics, biology, chemistry and information processing et al.. Two kinds of methods of identifying and distinguishing single-molecule are analyzed and reviewed. Firstly, two identification methods to identify singlemolecule photon source are introduced: one by measuring laser stimulated single-molecule fluorescence field twoorder autocorrelation function with start-stop method, the other based on single photon counting statistic measurement. Secondly, the other kind of methods are to distinguish which species molecules the single-molecule detected belongs to when a couple of species molecules are mixed. Two methods, based on property-correlated single-photon counting statistic measurement and probability calculation and analysis, are chiefly introduced: the maximum likelihood method and the lookup table method.
Laser & Optoelectronics Progress•Jan 1, 2006•0 citations