The Stark shift of the (6s
)2 1S0\ensuremath{\rightarrow}(6s6p
)1P1 transition in barium was measured using a laser to excite an atomic beam as it traversed a uniform electric field and a field-free region. The laser frequency was scanned across the transition while fluorescence produced by the radiative decay of the excited state was detected. The frequency scan was calibrated using an acousto-optic modulator which frequency shifted the laser by a specified amount. The Stark-shift rate was found to be -28.532\ifmmode\pm\else\textpm\fi{}0.060 kHz/(kV/cm
)2. The hyperfine splitting of the
135,137Ba F=1/2 level relative to
138Ba of the (6s6p
)1P1 state was determined to be -549.47\ifmmode\pm\else\textpm\fi{}0.12 MHz, where the minus sign indicates that the
138Ba transition is at a lower frequency.