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arXiv:2609.06193

Cell Reproduction in a Dark Optical Trap

Ariel Hertz, Gabriel Dias, Carlos L. R. Fragoso, Anna De Falco, A. Z. Khoury, Thiago Guerreiro

physics.opticsphysics.bio-phquant-phSubmitted Sep 5, 2026• Announced Sep 8, 2026
arXivHTMLPDF

Abstract

Optical tweezers are a versatile tool in the domain of cytology, enabling trapping and manipulation of individual cells. However, the incidence of laser light causes photodamage to biological matter, even when operating at low optical powers and over short periods of time. Here, we demonstrate stable trapping of single living Saccharomyces cerevisiae yeast cells in vitro using a dark optical trap operating in the repulsive regime of light-matter interactions. In contrast to standard tweezers, our dark optical trap confines cells for hours with negligible disruption to their morphology and reproduction cycle, opening up new possibilities for long duration experiments with living organisms such as the observation of cell reproduction under laser trapping.

Comments
8 pages, 7 figures, 1 table. Supplemental Material (3 movies) submitted separately

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Optical tweezers are a versatile tool in the domain of cytology, enabling trapping and manipulation of individual cells. However, the incidence of laser light causes photodamage to biological matter, even when operating at low optical powers and over short periods of time. Here, we demonstrate stable trapping of single living Saccharomyces cerevisiae yeast cells in vitro using a dark optical trap operating in the repulsive regime of light-matter interactions. In contrast to standard tweezers, our dark optical trap confines cells for hours with negligible disruption to their morphology and reproduction cycle, opening up new possibilities for long duration experiments with living organisms such as the observation of cell reproduction under laser trapping.
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Optical tweezers have become powerful tools for the confinement and manipulation of neutral atoms, molecules, mesoscopic biological molecules and living cells. In our experiment, a single caesium atom was prepared in a large-magnetic-gradient magneto-optical trap (MOT). It was then efficiently transferred back and forth between the MOT and a 1064 nm microscopic optical tweezer. The atomic transfer between the MOT and the tweezer can be employed to measure the trapping lifetime and the energy distribution of the single atom in the tweezer. In order to extend the trapping lifetime, laser cooling is used to decrease the atom's kinetic energy. The trapping lifetime was extended from ∼75 to ∼130 s by applying a 10 ms laser cooling phase just after the single atom is transferred into the tweezer.
We propose a new scheme for constructing a single-beam dark optical trap that minimizes light-induced perturbations of the trapped atoms. The proposed scheme optimizes the trap depth for given trapping laser power and detuning by creating a light envelope with (a) an almost minimal surface area for a given volume and (b) the minimal wall thickness that is allowed by diffraction. The stiffness of the trap’s walls, combined with the large detuning allowed by the efficient distribution of light intensity, yields a low spontaneous photon scattering rate for the trapped atoms. Our trap also optimizes the loading efficiency by maximizing the geometrical overlap between a magneto-optical trap and the dipole trap. We demonstrate this new scheme by generating the proposed light distribution of a single-beam dark trap with a trap depth that is ∼33 times larger than that of existing blue-detuned traps and ∼13 times larger than that of a red-detuned trap with the same diameter, detuning, and laser power. Trapped atoms are predicted to have a decoherence rate that is >200 times smaller than in existing single-beam dark traps and ∼1800 times smaller than in a red-detuned trap with the same diameter, depth, and laser power.
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