Quantum-limited estimation of the axial separation of two incoherent point sources
Yiyu Zhou, Jing Yang, Jeremy D. Hassett, Seyed Mohammad Hashemi Rafsanjani, Mohammad Mirhosseini, A. Nick Vamivakas, Andrew N. Jordan, Zhimin Shi, Robert W. Boyd
Improving axial resolution is crucial for three-dimensional optical imaging systems. Here we present a scheme of axial superresolution for two incoherent point sources based on spatial mode demultiplexing. A radial mode sorter is used to losslessly decompose the optical fields into a radial mode basis set to extract the phase information associated with the axial positions of the point sources. We show theoretically and experimentally that, in the limit of a zero axial separation, our scheme allows for reaching the quantum Cramér-Rao lower bound and thus can be considered as one of the optimal measurement methods. Unlike other superresolution schemes, this scheme does not require neither activation of fluorophores nor sophisticated stabilization control. Moreover, it is applicable to the localization of a single point source in the axial direction. Our demonstration can be useful to a variety of applications such as far-field fluorescence microscopy.
Improving axial resolution is crucial for three-dimensional optical imaging systems. Here we present a scheme of axial superresolution for two incoherent point sources based on spatial mode demultiplexing. A radial mode sorter is used to losslessly decompose the optical fields into a radial mode basis set to extract the phase information associated with the axial positions of the point sources. We show theoretically and experimentally that, in the limit of a zero axial separation, our scheme allows for reaching the quantum Cramér-Rao lower bound and thus can be considered as one of the optimal measurement methods. Unlike other superresolution schemes, this scheme does not require neither activation of fluorophores nor sophisticated stabilization control. Moreover, it is applicable to the localization of a single point source in the axial direction. Our demonstration can be useful to a variety of applications such as far-field fluorescence microscopy.
According to Rayleigh's criterion, two incoherent emitters with a separation below the diffraction limit are not resolvable with a conventional fluorescence microscope. One method of superresolution microscopy (SRM) circumvents the diffraction-limited resolution by precisely estimating the position of spatiotemporally independent emitters. However, these methods of SRM techniques are not optimal for estimating the separation of two simultaneously excited emitters. Recently, a number of detection methods based on modal imaging have been developed to achieve the quantum Cramér-Rao lower bound (QCRB) to estimate the separations between two nearby emitters. The QCRB determines the minimum achievable precision for all possible detection methods. Current modal imaging techniques assume a scalar field generated from a point source, such as a distant source from an optical fiber or a pinhole. However, for fluorescently labeled samples, point emitters are single fluorophores that are modeled as dipole emitters and, in practice, are often freely rotating. Dipole radiation must be described by vectorial theory, and the assumption of a scalar field no longer holds. Here, we present a method to numerically calculate the QCRB for measuring the separation of two dipole emitters, incorporating the vectorial theory. Furthermore, we propose a near-quantum optimal detection scheme based on one of the modal imaging techniques, superlocalization by image inversion interferometry (SLIVER), for estimating the separation of two freely rotating dipoles. In the proposed method, we introduce a vortex wave plate before the SLIVER detection to separate the radial and azimuthal components of the dipole radiation. With numerical simulations, we demonstrated that our method achieves nondivergent precision at any separation between two dipole emitters. We investigated several practical effects relevant to experimental measurements in superresolution microscopy, including numerical aperture, detection bandwidth, number of estimation parameters, background, and misalignment on separation estimation. Our proposed measurement provides a near-quantum-limited detection scheme for measuring the separation of two freely rotating dipole emitters, such as fluorescently tagged molecules, which are commonly used in superresolution microscopy.
Optical measurements that can achieve the fundamental quantum limits have the potential to improve the imaging of subdiffraction objects in important applications, including optical astronomy and fluorescence microscopy. Working towards the goal of implementing such quantum-inspired measurements for real applications, we experimentally demonstrate the localization of two incoherent optical point sources and the semiparametric estimation of object moments in the subdiffraction regime via spatial-mode demultiplexing (SPADE). In the case of two sources, we are able to estimate both of their locations accurately, not just their separation, by exploiting the asymmetric response of our SPADE device. In the case of semiparametric estimation, we demonstrate that, even if the source number is unknown, the moments of the source distribution can still be estimated accurately. Our demonstration paves the way towards the use of SPADE for optical superresolution in practical scenarios, where adaptive measurements are difficult and many parameters are unknown.
Resolving light sources below the diffraction limit is a fundamental task both for astronomy and microscopy. Several recent works analysed this problem through the lens of quantum parameter estimation theory and proved that the separation between two point sources can be estimated at the quantum limit using intensity measurements after spatial-mode demultiplexing. We implement this technique and provide an optimal estimator based on a linear combination of demultiplexed intensity measurements [1]–[3]. Our experimental setup (see Fig. 1 (left)) allows for the generation of the images of two sources, with tunable mutual coherence, as well as for spatial mode demultiplexing to estimate their separation [4].
We experimentally demonstrate the simultaneous estimation of the three parameters characterizing a pair of incoherent optical sources in the sub-Rayleigh regime, enabling super-resolved scene characterization. Using spatial-mode demultiplexing (SPADE) with two demultiplexers--one deliberately shifted--we determine separations well below the diffraction limit and achieve sensitive joint estimation of separation, centroid, and relative brightness over a broad range of scene configurations in a single experimental setting. We benchmark our performance using Fisher-information-based Cramér-Rao bounds, and discuss the corresponding quantum limits. We investigate two complementary scenarios: a realistic case with slightly non-identical sources, and an idealized case of indistinguishable sources.
Resolving light sources below the diffraction limit is a fundamental task both for astronomy and microscopy. Several recent works analysed this problem through the lens of quantum parameter estimation theory and proved that the separation between two point sources can be estimated at the quantum limit using intensity measurements after spatial-mode demultiplexing. We implement this technique and provide an optimal estimator based on a linear combination of demultiplexed intensity measurements [1–3]. Our experimental setup allows for the generation of the images of two sources, with tunable mutual coherence, as well as for spatial mode demultiplexing to estimate their separation [4].
For more than a century, the diffraction limit has defined the resolution achievable by passive optical imaging systems. Although some resolution improvement can be gained through classical data processing of the image, it is limited by the noise arising from quantum nature of light. Minimizing the effect of this noise requires quantum treatment of optical imaging. By reformulating imaging as a problem of quantum measurement and estimation, it becomes possible to identify optimal detection strategies that recover spatial information previously thought inaccessible. This review summarizes the theoretical framework that underpins this development, from the formulation of quantum Cramer-Rao bounds and Chernoff bounds to the construction of receivers that attain them, such as those based on spatial-mode demultiplexing. We show how these methods can beat conventional imaging in the classification, localization, and imaging of sub-Rayleigh incoherent sources. We then discuss extensions to multiparameter and partially coherent scenarios, and highlight the unifying connections between estimation and discrimination tasks. Finally, we survey recent experimental demonstrations that approach quantum-limited resolution and outline emerging applications in microscopy, astronomy, and optical sensing.