With the progress of optical detection technology, the classical diffraction limit raised a hundred years ago has been continuously broken through. In previous experiments within fluorescence sources, one of the techniques used is detecting auto-correlation functions. The image is reconstructed by single-photon and multi-photon detecting intensities. By taking the joint intensity of more than one single-photon detectors into consideration, the point spread function of each photon emitter can be resolved even when the distance of their central position is below the classical diffraction limit. However, the measurement precision is not considered. In actual detecting process the detectors are imperfect, they have quantum detecting efficiency $η$ and even can not count the photon numbers during one absorbing process. Therefore, we analyze the detecting intensity of each detector separately and use the total Fisher information to depict the resolution. Higher Fisher information is obtained when second order correlation function is considered and higher detecting efficiency is beneficial to the Fisher information enhancement, meanwhile the Cramèr-Rao bound for an effective distance resolving is lower than the classical diffraction limits. Furthermore, when the emitted photons are coherent states, the resolution ability can be enhanced with infinite single-photon detectors.
With the progress of optical detection technology, the classical diffraction limit raised a hundred years ago has been continuously broken through. In previous experiments within fluorescence sources, one of the techniques used is detecting auto-correlation functions. The image is reconstructed by single-photon and multi-photon detecting intensities. By taking the joint intensity of more than one single-photon detectors into consideration, the point spread function of each photon emitter can be resolved even when the distance of their central position is below the classical diffraction limit. However, the measurement precision is not considered. In actual detecting process the detectors are imperfect, they have quantum detecting efficiency η and even can not count the photon numbers during one absorbing process. Therefore, we analyze the detecting intensity of each detector separately and use the total Fisher information to depict the resolution. Higher Fisher information is obtained when second order correlation function is considered and higher detecting efficiency is beneficial to the Fisher information enhancement, meanwhile the Cramèr-Rao bound for an effective distance resolving is lower than the classical diffraction limits. Furthermore, when the emitted photons are coherent states, the resolution ability can be enhanced with infinite single-photon detectors.
The classical optical diffraction limit can be overcome by exploiting the quantum properties of light in several theoretical studies; however, they mostly rely on an entangled light source. Recent experiments have demonstrated that quantum properties are preserved in many fluorophores, which makes it possible to add a new dimension of information for super-resolution fluorescence imaging. Here, we developed a statistical quantum coherence model for fluorescence emitters and proposed a new super-resolution method using fluorescence quantum coherence in fluorescence microscopy. In this study, by exploiting a single-photon avalanche detector (SPAD) array with a time-correlated single-photon-counting technique to perform spatial-temporal photon statistics of fluorescence coherence, the subdiffraction-limited spatial separation of emitters is obtained from the determined coherence. We numerically demonstrate an example of two-photon interference from two common fluorophores using an achievable experimental procedure. Our model provides a bridge between the macroscopic partial coherence theory and the microscopic dephasing and spectral diffusion mechanics of emitters. By fully taking advantage of the spatial-temporal fluctuations of the emitted photons as well as coherence, our quantum-enhanced imaging method has the significant potential to improve the resolution of fluorescence microscopy even when the detected signals are weak.
The fundamental understanding of biological pathways requires minimally invasive nanoscopic optical resolution imaging. Many approaches to high-resolution imaging rely on localization of single emitters, such as fluorescent molecules or quantum dots. Additionally, the exact determination of the number of such emitters in an imaging volume is essential for a number of applications; however, in standard intensity-based microscopy it is not possible to determine the number of individual emitters within a diffraction limited spot without initial knowledge of system parameters. Here we explore how quantum measurements of the emitted photons using photon number resolving detectors can be used to address this challenging task. In the proposed new approach, the problem of counting emitters reduces to the task of determining differences between the emitted photon distribution and the Poisson limit. We show that quantum measurements of the number of photons emitted from an ensemble of emitters enable the determination of both the number of emitters and the probability of emission. This method can be applied for any type of single-photon emitters. The scaling laws of this new approach are presented by the Cramer-Rao Lower Bounds, and this technique has great potential in quantum optical imaging with nanoscopic resolution.
Quantum imaging emerged quite recently as a breakthrough technology of overcoming the diffraction limit in microscopy and enhancement of optical resolution without the necessity to use hard radiation or perform scanning in the near field [1] , [2] . Both, the quantum imaging itself and the more “classical” techniques inspired by it (for example, super-resolution optical fluctuations imaging – SOFI [3] ), rely on detection and analysis of photon (intensity) correlations. Typically, it is believed that the more correlated illuminating light is used and the higher order of the correlations is measured, the larger super-resolution can be achieved. That conclusion is based on efficient narrowing of the point-spread function, which, however, does not necessarily imply better resolution as the ability to reconstruct smaller features of the investigated object successfully.
Imaging is the estimation of the spatial distribution of a physical object by measuring the optical radiation it emits, or by making use of an optical wave that interacts with the object, via reflection or transmission, for example, before being measured by a detector. The resolution of an imaging system is limited by the inability to localize the optical field at points of the object. Under otherwise ideal conditions, resolution is limited by diffraction. The sensitivity of an imaging system is limited by the uncertainty in the measurement. Under ideal conditions, this is determined by photon noise, which depends on the statistical fluctuations of the light. In conventional imaging systems, an extended detector, such as a CCD camera or an array detector, measures the spatial distribution of the optical intensity, which is proportional to the photon flux density. In interferometric systems, the spatial distribution of the optical field is inferred from measurements of the optical intensity. With the emergence of coherence theory, imaging systems based on measurements of the second-order coherence function at pairs of points in the detection plane were developed. An example is the imaging of an incoherent object based on the van Cittert-Zernike theorem. Imaging systems based on measurement of intensity correlation, or the photon coincidence rate, at pairs of points, were developed in the 1960s. A classic example of the photon-correlation imaging of an object emitting thermal light is stellar imaging using a Hanbury-Brown-Twiss intensity-correlation interferometer. More recently, two-photon light, which may be generated via spontaneous parametric downconversion in a second-order nonlinear optical crystal, has been used for imaging. This type of two-photon (or biphoton) imaging, which has come to be called quantum imaging, is also based on the measurement of photon coincidence by the use of photon-counting array detectors or by scanning two photon-counting detectors at pairs of points. To compare the resolution and sensitivity of imaging systems based on the aforementioned types of measurements, it is necessary to derive expressions for the measured quantities in terms of the object distribution. The point-spread functions based on such expressions can be used to assess the resolution. One measure of the sensitivity of the imaging process is the signal-to-noise ratio (SNR) of the measured variables. The statistical nature of the light source must be known in order to determine the SNR. The purpose of this chapter is to compare the resolution and sensitivity of photon-correlation imaging systems that make use of thermal light and two-photon light. We will henceforth refer to these two imaging modalities as classical and quantum photon-correlation imaging, or simply classical and quantum imaging, respectively. Clearly, light in other quantum states can also be used for imaging.
The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in optical imaging using quantum properties of light. An experimental realization of sub-diffraction limited quantum imaging has, however, remained elusive. Here, we demonstrate a quantum imaging method taking advantage of non-classical light naturally produced in fluorescence microscopy due to photon antibunching, a fundamentally quantum phenomenon prohibiting simultaneous emission of multiple photons. Using a photon counting digital camera, we detect antibunching-induced second and third order intensity correlations and perform sub-diffraction limited quantum imaging in a standard wide-field fluorescence microscope.
Precision measurement has been an important research area in sensing and metrology. In classical physics, the Fisher information determines the maximum extractable information from statistically unknown signals, based on a joint probability density function of independently and identically distributed random variables. The Cramer-Rao lower bound (CRLB) indicates the minimum error of the Fisher information, generally known as the shot-noise limit. On the other hand, coherence has pushed the resolution limit further overcoming the diffraction limit using many-wave interference strictly confined to the first-order intensity correlation. However, practical implementation is limited by the lithographic constraints in, e.g., optical gratings. Recently, a coherence technique of superresolution has been introduced to overcome the diffraction limit in phase sensitivity using higher-order intensity correlations of a phase-controlled output field from an interferometer. Here, the superresolution is adopted for precision metrology in an optical spectrometer, whose enhanced frequency resolution is linearly proportional to the intensity-product order, overcoming CRLB. Unlike quantum sensing using entangled photons, this technique is purely classical and offers robust performance against environmental noises, benefiting from the interferometer's scanning mode for fringe counting.