We report on the realization of large assembled arrays of more than 300 single
87Rb atoms trapped in optical tweezers in a cryogenic environment at $\ensuremath{\sim}4$ K. For arrays with
Na=324 atoms, the assembly process results in defect-free arrays in $\ensuremath{\sim}37%$ of the realizations. To achieve this high assembling efficiency, we equalize the loading probability of the traps within the array using a closed-loop optimization of the power of each optical tweezer, based on the analysis of the fluorescence time traces of atoms loaded in the traps.