We present a dual-region experimental apparatus for trapping single atoms of the fermionic isotope ytterbium-171 (
171Yb) with optical tweezers in a science chamber containing an in-vacuum nanofiber cavity. A source region is located approximately
240mm from the science chamber, preserving optical access around the chamber for high-numerical-aperture tweezer optics. A pulsed transport sequence based on longitudinal moving molasses launches atomic pulses toward the science chamber, where they are decelerated and recaptured in a magneto-optical trap. We subsequently load single atoms into a
2×2 tweezer array located approximately
50μm from the nanofiber and measure a
1/e lifetime of
9.5(7)s. These results demonstrate that the dual-region architecture provides sufficient
171Yb atoms for single-atom tweezer operation near an in-vacuum nanofiber cavity.