We simultaneously confine fermionic metastable
3He atoms and bosonic metastable
4He atoms in a magneto-optical trap. The trapped clouds, containing up to $1.5\ifmmode\times\else\texttimes\fi{}{10}^{8}$ atoms of each isotope, are characterized by measuring ions and metastable helium atoms escaping from the trap. Optical pumping of
3He atoms to a nontrapped hyperfine state is investigated and it is shown that large atom numbers can be confined without additional repumping lasers. Unique possibilities for quantum degeneracy experiments with mixtures of spin-polarized metastable
3He and
4He atoms are indicated.
This thesis presents experimental and theoretical work performed at the Laser Centre of the Vrije Universiteit in Amsterdam to study laser-cooled metastable triplet helium atoms. Samples containing about 3x10^8 helium atoms-either fermionic helium-3 atoms, bosonic helium-4 atoms or mixtures thereof-are cooled to a temperature around 1 mK and form the starting point of the presented studies. The studies include an investigation of cold ionizing collisions in the absence of resonant light, an investigation of magneto-optical trapping with ultraviolet light, a feasibility study of an atomic fountain clock based on metastable helium-3 atoms and the first demonstration of magneto-optical trapping of an isotopic mixture of metastable helium atoms.