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Multiphoton Microscopy in the Biomedical Sciences

Nonlinear Raman and second harmonic imaging with quantum light (Conference Presentation)

Vladislav V. Yakovlev

Published Mar 4, 2019• 0 citations
Publisher

Abstract

Advanced spectroscopic tools are now widely utilized in microscopic optical imaging. Most of those techniques have already reached the level of “quantum limited” detection using classical light. In this report I will explored quantum-optical approaches to microscopic optical imaging. Using two selective examples of Raman and second-harmonic imaging, I will illustrate potential advantages of such approaches and discuss potential challenges and instrumentation developments.

DOI
10.1117/12.2510929

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Advanced spectroscopic tools are now widely utilized in microscopic optical imaging. Most of those techniques have already reached the level of “quantum limited” detection using classical light. In this report I will explored quantum-optical approaches to microscopic optical imaging. Using two selective examples of Raman and second-harmonic imaging, I will illustrate potential advantages of such approaches and discuss potential challenges and instrumentation developments.
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We explore the use of nonlinear optical methods for important applications in quantum information science. One example is the use nonlinear optical methods in the context of “quantum imaging.” We describe some of the conceptual possibilities afforded by the marriage of quantum and classical imaging methods, and we describe recent work that illustrates some of these features. We also address the complementary issue of how advanced imaging protocols can allow one to utilize and manipulate the enormous information content residing in the transvers structure of a quantum light field.
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Microscopes are indispensable tools in modern biology and medicine.With the development of microscopy, the signal-tonoise ratio of microscopes is now limited by the shot noise.Recently, quantum-enhanced microscopic imaging provides a feasible approach for improving the signal-to-noise ratio since it can beat the shot-noise limit by using quantum light.In this review, we first briefly introduce quantum states applied in quantum-enhanced microscopic imaging, and then we provide an overview of the principle and progress of quantum-enhanced stimulated Raman scattering microscopy, entangled twophoton microscopy, and quantum-enhanced differential interference contrast microscopy.