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Conference on Lasers and Electro-Optics

Single-photon fiber bundle cameras (SFICAMs) for quantum enhanced superresolution microscopy

Yonatan Israel, Ron Tenne, Dan Oron, Yaron R. Silberberg

Published Jan 1, 2017• 0 citations
Publisher

Abstract

We present a method that utilizes quantum correlation measurements for multi-emitter sub-diffraction localization in a time-dependent scene. This is demonstrated using a newly developed imaging configuration based on fiber bundle coupled single-photon avalanche detectors.
DOI
10.1364/cleo_qels.2017.ftu3f.7

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The optical diffraction limit imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in optical imaging using quantum properties of light. Here, we demonstrate a quantum superresolution imaging method taking advantage of nonclassical light naturally produced in fluorescence microscopy due to photon antibunching, a fundamentally quantum phenomenon inhibiting simultaneous emission of multiple photons. Using a photon counting digital camera, we detect antibunching-induced second and third order intensity correlations and perform subdiffraction limited quantum imaging in a standard wide-field fluorescence microscope.
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The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in optical imaging using quantum properties of light. An experimental realization of sub-diffraction limited quantum imaging has, however, remained elusive. Here, we demonstrate a quantum imaging method taking advantage of non-classical light naturally produced in fluorescence microscopy due to photon antibunching, a fundamentally quantum phenomenon prohibiting simultaneous emission of multiple photons. Using a photon counting digital camera, we detect antibunching-induced second and third order intensity correlations and perform sub-diffraction limited quantum imaging in a standard wide-field fluorescence microscope.
Abstract Optical superresolution microscopy is an important field, where nonlinear optical processes or prior information is used to defeat the classical diffraction limit of light. Quantum correlation microscopy uses photon arrival statistics from single photon emitters to aid in the determination of properties including the number of emitters and their relative brightness. Here we model quantum correlation microscopy in the few emitter regime, i.e. around four single photon emitters below the diffraction limit. We use the Akaike Information Criterion to determine the number of emitters and we vary the relative contributions of intensity to quantum correlation information to determine contribution that provides optimal imaging. Our results show diffraction unlimited performance and a change in localisation scaling behaviour dependent on emitter closeness.