We show that a quaternionic quantum field theory can be formulated when the numbers of bosonic and fermionic degrees of freedom are equal and the fermions, as well as the bosons, obey a second-order wave equation. The theory is initially defined in terms of a quaternion-imaginary Lagrangean using the Feynman sum over histories. A Schr\"odinger equation can be derived from the functional integral, which identifies the quaternion-imaginary quantum Hamiltonian. Conversely, the transformation theory based on this Hamiltonian can be used to rederive the functional-integral formulation.