The quantum relative R\'enyi entropy of two noncommuting density matrices was recently defined from which its conditional entropy is deduced. This framework is here extended to the corresponding Tsallis relative entropy and to its conditional form. This expression of Tsallis conditional entropy is shown to witness entanglement beyond the method based on global and local spectra of composite quantum states. When the reduced density matrix happens to be a maximally mixed state, this conditional entropy coincides with the expression in terms of Tsallis entropies derived earlier by Abe and Rajagopal [Physica A 289, 157 (2001)]. The separability range in a noisy one-parameter family of
W and Greenberger-Horne-Zeilinger states with three and four qubits is explored here and it is shown that the results inferred from negative Tsallis conditional entropy matches that obtained through Peres's partial transpose criterion for one-parameter family of
W states, in one of its partitions. The criterion is shown to be nonspectral through its usefulness in identifying entanglement in isospectral density matrices.